Difference between revisions of "Part:BBa K4438127"
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<partinfo>BBa_K4438127 short</partinfo> | <partinfo>BBa_K4438127 short</partinfo> | ||
| − | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438107, with BBa_J64997 upstream and BBa_K731721 downstream, along with the spacer sequences BBa_K3831027(at 5’ end) and BBa_K4438136(at 3’end). FASTmiR-146a-D3_s, after transcription, binds to miR-146a, the transcription product of | + | FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for <partinfo>BBa_K4438107</partinfo>, with <partinfo>BBa_J64997</partinfo> upstream and <partinfo>BBa_K731721</partinfo> downstream, along with the spacer sequences <partinfo>BBa_K3831027</partinfo>(at 5’ end) and <partinfo>BBa_K4438136</partinfo>(at 3’end). FASTmiR-146a-D3_s, after transcription, binds to miR-146a, the transcription product of <partinfo>BBa_K4438151<partinfo>. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part can be used for the detection of miR-146a, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The | + | This part can be used for the detection of miR-146a, which proves to be a biomarker for many diseases. A promoter sequence added in the beginning enhances its usage in both in-vivo and in-vitro conditions. The OFF(A) and ON(B) structure for the sensor was generated as given: |
| − | + | ||
| + | [[File:T--IISER-Tirupati_India--miRNA_146_3.png]] | ||
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Revision as of 09:45, 12 October 2022
FASTmiR-146a-D3_s
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This part is the expression cassette for BBa_K4438107, with BBa_J64997 upstream and BBa_K731721 downstream, along with the spacer sequences BBa_K3831027(at 5’ end) and BBa_K4438136(at 3’end). FASTmiR-146a-D3_s, after transcription, binds to miR-146a, the transcription product of No part name specified with partinfo tag.
Notes
The split position of the complementary sequence in the sensor is CTGAAGAACT….GAATTTCAGAGG
References
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130