Difference between revisions of "Part:BBa K4121052"
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The composite part is called "pFBA1-TmCrtE-tTDH1". We use pFBA1 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).
 
The composite part is called "pFBA1-TmCrtE-tTDH1". We use pFBA1 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).
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===Design Notes===
 
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We used the "Goldengate" method for the ligation of the basic parts, We introduced recognition sites and cleavage sites for the type IIs restriction endonuclease BsmBI at both ends of the Basic Parts to ensure that the Basic Parts could be ligated successfully, and changed the location of the recognition sites and cleavage sites so that the BsmBI would not cut repeatedly.
 
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To avoid homologous recombination in Saccharomyces cerevisiae, we substitute different promoters and terminators.
 
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Please refer to" Design" part of the Wiki for detailed experimental design.
 
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===Source===
 
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We get sequences of the promoter from Eukaryotic promoter database(EPD), the sequences of CDS and the terminator from National Center for Biotechnology Information(NCBI).
 
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===Usage and Biology===
 
===Usage and Biology===
  
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<partinfo>BBa_K4121052 SequenceAndFeatures</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4121006 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4121052 parameters</partinfo>
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<partinfo>BBa_K4121006 parameters</partinfo>
 
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Revision as of 09:41, 12 October 2022


Expression cassette of TmCrtE with constitutive promoter

The composite part is called "pFBA1-TmCrtE-tTDH1". We use pFBA1 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 451
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 451
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 451
    Illegal XhoI site found at 399
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 451
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 451
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 493