Difference between revisions of "Part:BBa K176229:Design"
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<partinfo>BBa_K176229 short</partinfo> | <partinfo>BBa_K176229 short</partinfo> | ||
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Input1 PoPS generates LuxI-LVA and TetR-LVA. | Input1 PoPS generates LuxI-LVA and TetR-LVA. | ||
| − | pCon 0.15(K176008) generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. | + | pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density. |
pCon 0.70(J23101) generates LuxR. | pCon 0.70(J23101) generates LuxR. | ||
Latest revision as of 14:21, 13 October 2009
High input score automatic selector: PoPS->luxI-LVA->K176226
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1524
Illegal NheI site found at 1547
Illegal NheI site found at 2373
Illegal NheI site found at 2396 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 655
Illegal BglII site found at 2215 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3840
Illegal BsaI.rc site found at 3351
Design Notes
PoPS->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)
Input1: PoPS
Input2: AHL
Output1: AHL
Output2: Cell Density
Design:
Input1 PoPS generates LuxI-LVA and TetR-LVA.
pCon 0.15(K176008) also generates LuxI-LVA, LuxI-LVA generates Output1 AHL, the concentration of AHL in the medium depends on both LuxI-LVA level per cell and cell density.
pCon 0.70(J23101) generates LuxR.
pLux/Tet(K176000) generates lacZalpha-ccdB (weak RBS) to kill the cell, activated by LuxR and (Output1 AHL generated by LuxI-LVA + Input2 AHL), repressed by TetR-LVA and no aTc.
Source
C0261 K176226