Difference between revisions of "Part:BBa K4121051"
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The composite part is called "pCCW12-TmCrtE-tTDH1". We use pCCW12 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).
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__NOTOC__
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<partinfo>BBa_K4121051 short</partinfo>
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Expression cassette of TmCrtE with constitutive promoter
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The composite part is called "pCCW12-TmCrtE-tTDH1". We use pCCW12 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4121051 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4121051 parameters</partinfo>
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Revision as of 09:30, 12 October 2022


Expression cassette of TmCrtE with constitutive promoter

Expression cassette of TmCrtE with constitutive promoter

The composite part is called "pCCW12-TmCrtE-tTDH1". We use pCCW12 as the promoter, tTDH1 as the terminator and the CDS of this part is TmCrtE(from Taxus media). The three basic parts above make up a new transcription unit, it has the fuction to catalyze the conversion of farnesyl pyrophosphate (FPP) to heterologous geranylgeranyl diphosphate(GGPP).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
    Illegal BglII site found at 589
    Illegal BglII site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
    Illegal XbaI site found at 727
  • 1000
    COMPATIBLE WITH RFC[1000]