Difference between revisions of "Part:BBa K4236102"
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The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS, and UGT. We want to construct a big plasmid consisting of three genes we have introduced to produce astragalin. In order to better express each enzyme, we tried to connect the smaller F3H and FLS with a linker to form a fusion protein, and the larger UGT was expressed separately. Therefore, AtF3H-AtFLS + AtUGT expression vector was constructed. | The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS, and UGT. We want to construct a big plasmid consisting of three genes we have introduced to produce astragalin. In order to better express each enzyme, we tried to connect the smaller F3H and FLS with a linker to form a fusion protein, and the larger UGT was expressed separately. Therefore, AtF3H-AtFLS + AtUGT expression vector was constructed. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 09:09, 12 October 2022
T7-RBS-atF3H-atFLS+atUGT
The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS, and UGT. We want to construct a big plasmid consisting of three genes we have introduced to produce astragalin. In order to better express each enzyme, we tried to connect the smaller F3H and FLS with a linker to form a fusion protein, and the larger UGT was expressed separately. Therefore, AtF3H-AtFLS + AtUGT expression vector was constructed.
Usage and Biology
Other Information
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]