Difference between revisions of "Part:BBa K4477006:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to get a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene.
+
We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477001 without overburdening the <em>E. coli</em> expressing the gene.
  
 
We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.
 
We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.

Latest revision as of 08:35, 12 October 2022


RBS for expression of human/mouse anti-apoB(MDA) antibody light chain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used a pre-existing RBS, BBa_J61100, as a starting point for Salis optimization. We aimed for 75% relative expression so as to achieve a high level of protein expression of BBa_K4477001 without overburdening the E. coli expressing the gene.

We adjusted the RBS sequence to remove restriction sites that would make the RBS incompatible with various assembly methods.

Source

https://salislab.net/software/

References