Difference between revisions of "Part:BBa K4117888:Design"
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We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503 | We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503 | ||
| − | + | anti-THC pmrB gene come from Part:[https://parts.igem.org/Part:BBa_K4117779, BBa_K4117779.] | |
| − | The pmrC promoter comes from Part:BBa_K1459006. | + | The pmrC promoter comes from Part:[https://parts.igem.org/Part:BBa_K1459006, BBa_K1459006.] |
| − | The pmrA gene comes from Part:BBa_K3611003. | + | The pmrA gene comes from Part:[https://parts.igem.org/Part:BBa_K3611003, BBa_K3611003.] |
| − | The ompT signal peptide gene comes from Part:BBa_K3805532. | + | The ompT signal peptide gene comes from Part:[https://parts.igem.org/Part:BBa_K3805532, BBa_K3805532.] |
| − | The ompA signal peptide gene comes from Part:BBa_K103006. | + | The ompA signal peptide gene comes from Part:[https://parts.igem.org/Part:BBa_K103006, BBa_K103006.] |
| − | T7 terminator gene comes from Part:BBa_B0012. | + | T7 terminator gene comes from Part:[https://parts.igem.org/Part:BBa_B0012, BBa_B0012.] |
| − | The rrnB T1 Terminator gene comes from BBa_K3257020. | + | The rrnB T1 Terminator gene comes from [https://parts.igem.org/Part:BBa_K3257020, BBa_K3257020.] |
===References=== | ===References=== | ||
Latest revision as of 08:34, 12 October 2022
UGT1A3
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4599
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4599
Illegal BglII site found at 4257
Illegal BamHI site found at 2887
Illegal BamHI site found at 3444 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal AgeI site found at 3031 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 732
Design Notes
To degrade Δ9-THC, we added a pathway to sense Δ9-THC in front of the UGT1A3 gene.
Source
We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503
anti-THC pmrB gene come from Part:BBa_K4117779.
The pmrC promoter comes from Part:BBa_K1459006.
The pmrA gene comes from Part:BBa_K3611003.
The ompT signal peptide gene comes from Part:BBa_K3805532.
The ompA signal peptide gene comes from Part:BBa_K103006.
T7 terminator gene comes from Part:BBa_B0012.
The rrnB T1 Terminator gene comes from BBa_K3257020.
References
[1] Sinsinbar G, Gudlur S, Metcalf KJ, Mrksich M, Nallani M, Liedberg B. Role of Lipopolysaccharide in Protecting OmpT from Autoproteolysis during In Vitro Refolding. Biomolecules. 2020;10(6):922. Published 2020 Jun 18.Ct
[2] Sugimura K, Nishihara T. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. J Bacteriol. 1988 Dec;170(12):5625-32. doi: 10.1128/jb.170.12.5625-5632.1988. PMID: 3056908; PMCID: PMC211661.
[3] Mazur A, Lichti CF, Prather PL, Zielinska AK, Bratton SM, Gallus-Zawada A, Finel M, Miller GP, Radomińska-Pandya A, Moran JH. Characterization of human hepatic and extrahepatic UDP-glucuronosyltransferase enzymes involved in the metabolism of classic cannabinoids. Drug Metab Dispos. 2009 Jul;37(7):1496-504. doi: 10.1124/dmd.109.026898. Epub 2009 Apr 1. PMID: 19339377; PMCID: PMC2698943.