Difference between revisions of "Part:BBa K4244014"
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<partinfo>BBa_K4244014 short</partinfo> | <partinfo>BBa_K4244014 short</partinfo> | ||
| − | sfGFP <partinfo>BBa_K4244004</partinfo> under an inducible promoter <partinfo>BBa_K914003</partinfo>. For better titration using <partinfo>BBa_K914003</partinfo> we introduced the plasmid in a Δ ''rhaB'',Δ ''rhaT'' strain | + | sfGFP <partinfo>BBa_K4244004</partinfo> under an inducible promoter <partinfo>BBa_K914003</partinfo>. For better titration using <partinfo>BBa_K914003</partinfo> we introduced the plasmid in a Δ ''rhaB'',Δ ''rhaT'' strain [1]. This construct was used as a positive control for <partinfo>BBa_K4244003</partinfo>, <partinfo>BBa_K4244005</partinfo>,<partinfo>BBa_K4244026</partinfo>, <partinfo>BBa_K4244012</partinfo>, <partinfo>BBa_K4244013</partinfo>. |
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Revision as of 08:28, 12 October 2022
Rhamose inducible sfGFP
sfGFP BBa_K4244004 under an inducible promoter BBa_K914003. For better titration using BBa_K914003 we introduced the plasmid in a Δ rhaB,Δ rhaT strain [1]. This construct was used as a positive control for BBa_K4244003, BBa_K4244005,BBa_K4244026, BBa_K4244012, BBa_K4244013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
1. Hjelm A, Karyolaimos A, Zhang Z, Rujas E, Vikström D, Slotboom DJ, et al. Tailoring Escherichia coli for the l -Rhamnose PBAD Promoter-Based Production of Membrane and Secretory Proteins. ACS Synth Biol. 2017;6(6):985–94.