Difference between revisions of "Part:BBa K4201017:Design"

 
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===Design Notes===
 
===Design Notes===
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Part information and design consideration can be found on respective parts pages.  
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Part information and design consideration can be found on respective basic parts pages.
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This construct differs from similar composite parts like BBa_K4201016 and BBa_K4201018 by having different promoter (Gmubi) and terminator (AtHSP) amounts and locations. In these constructs, dashes(-) represent locations where genes were synthesized <i>de novo</i>. An underscore (_) symbolizes a location where we adhered genes together via Golden Gate assembly. Utilizing Golden Gate assembly allowed us to insert promoter and terminator sequences between genes of interest, and consequently enables the study of transcription efficacy based on promoter and terminator location.
  
 
===Source===
 
===Source===

Revision as of 08:12, 12 October 2022


CrtE_cytoTDS2_RUBY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 10846
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2087
    Illegal PstI site found at 5638
    Illegal PstI site found at 10846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
    Illegal BglII site found at 1153
    Illegal BglII site found at 2021
    Illegal BglII site found at 2956
    Illegal BglII site found at 4011
    Illegal BglII site found at 5324
    Illegal BglII site found at 5572
    Illegal BamHI site found at 9058
    Illegal XhoI site found at 3601
    Illegal XhoI site found at 3868
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 948
    Illegal BsaI site found at 1887
    Illegal BsaI site found at 2167
    Illegal BsaI site found at 3113
    Illegal BsaI site found at 5438
    Illegal BsaI site found at 5718
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1873
    Illegal BsaI.rc site found at 2153
    Illegal BsaI.rc site found at 3100
    Illegal BsaI.rc site found at 5424
    Illegal BsaI.rc site found at 5704
    Illegal BsaI.rc site found at 6651


Design Notes

Part information and design consideration can be found on respective basic parts pages.

This construct differs from similar composite parts like BBa_K4201016 and BBa_K4201018 by having different promoter (Gmubi) and terminator (AtHSP) amounts and locations. In these constructs, dashes(-) represent locations where genes were synthesized de novo. An underscore (_) symbolizes a location where we adhered genes together via Golden Gate assembly. Utilizing Golden Gate assembly allowed us to insert promoter and terminator sequences between genes of interest, and consequently enables the study of transcription efficacy based on promoter and terminator location.

Source

Gmubi promoters originate from Glycine max.

AtHSP terminators originate from the Arabidopsis thaliana genome.

CrtE is a GGPP synthase from Pantoea ananatis LMG 20103

cytoTDS2 is a taxadiene synthase native to Taxus chinensis var. mairei optimized for use in Glycine max.

RUBY is a reporter gene from the order Caryophyllales.


References