Difference between revisions of "Part:BBa K4325003"
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<i> gshF </i>encodes a bifunctional glutathione synthetase GshF originated from <i> Streptococcus thermophilus</i> | <i> gshF </i>encodes a bifunctional glutathione synthetase GshF originated from <i> Streptococcus thermophilus</i> | ||
===Usage=== | ===Usage=== | ||
| − | <p>it is less sensitive to GSH, is selected and codon optimized for expressing in <i>G. hansenii</i>. | + | <p>it is less sensitive to GSH, is selected and codon optimized for expressing in <i>G. hansenii</i>. |
| − | + | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
| Line 21: | Line 20: | ||
1. Verification of Western blot electrophoresis in <i> E. coli</i>.</h4> | 1. Verification of Western blot electrophoresis in <i> E. coli</i>.</h4> | ||
[[File:Mfold-K3257013-1.png|600px|thumb|center|Figure1: Verification of Western blot electrophoresis in <i> E. coli.</i>]] | [[File:Mfold-K3257013-1.png|600px|thumb|center|Figure1: Verification of Western blot electrophoresis in <i> E. coli.</i>]] | ||
| − | + | As shown in Figure 1, the expression of GshF were verified successfully by Western blot.</p> | |
<h4>2.References</h4> | <h4>2.References</h4> | ||
<p>[1]Li W1,Li Z,Yang J,Ye Q, et al.Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli.Journal of Biotechnology, 12 Jun 2011, 154(4):261-268.</p> | <p>[1]Li W1,Li Z,Yang J,Ye Q, et al.Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli.Journal of Biotechnology, 12 Jun 2011, 154(4):261-268.</p> | ||
Revision as of 08:11, 12 October 2022
gshF
Description
gshF encodes a bifunctional glutathione synthetase GshF originated from Streptococcus thermophilus
Usage
it is less sensitive to GSH, is selected and codon optimized for expressing in G. hansenii.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
Illegal NotI site found at 1838
Illegal NotI site found at 2038 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal XhoI site found at 2019 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 178
Illegal EcoRI site found at 760
Illegal EcoRI site found at 1195
Illegal EcoRI site found at 1693
Illegal PstI site found at 19
Illegal PstI site found at 49
Illegal PstI site found at 406
Illegal NgoMIV site found at 1620
Illegal NgoMIV site found at 2251 - 1000COMPATIBLE WITH RFC[1000]
2022 SZPT-China
Characterization
1. Verification of Western blot electrophoresis in E. coli.
As shown in Figure 1, the expression of GshF were verified successfully by Western blot.
2.References
[1]Li W1,Li Z,Yang J,Ye Q, et al.Production of glutathione using a bifunctional enzyme encoded by gshF from Streptococcus thermophilus expressed in Escherichia coli.Journal of Biotechnology, 12 Jun 2011, 154(4):261-268.