Difference between revisions of "Part:BBa K4477004:Design"

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===Design Notes===
 
===Design Notes===
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Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle).
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Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle). Illegal restriction sites removed via codon optimization.
−
 
+
  
 +
For detailed annotations at to which nucleotides form the variable light, variable heavy, and linker sequence domains, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/
  
 
===Source===
 
===Source===

Revision as of 08:03, 12 October 2022


McPC603 anti-oxLDL scFv antibody fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle). Illegal restriction sites removed via codon optimization.

For detailed annotations at to which nucleotides form the variable light, variable heavy, and linker sequence domains, see Team Virginia 2022's Benchling folder here: https://benchling.com/mrkhoury/f_/YR4zZSUb-virginia-igem-2022-dna-constructs/

Source

1. https://www.sciencedirect.com/science/article/pii/S0769262585800581
2. https://pubmed.ncbi.nlm.nih.gov/14644097/

References