Difference between revisions of "Part:BBa K4477004:Design"

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===Source===
 
===Source===
  
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1. https://www.sciencedirect.com/science/article/pii/S0769262585800581 [sequence of IK17]<br>
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1. https://www.sciencedirect.com/science/article/pii/S0769262585800581<br>
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2. https://pubmed.ncbi.nlm.nih.gov/14644097/ [(G4S)3 sequence]
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2. https://pubmed.ncbi.nlm.nih.gov/14644097/
  
 
===References===
 
===References===

Revision as of 07:58, 12 October 2022


McPC603 anti-oxLDL scFv antibody fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle).


Source

1. https://www.sciencedirect.com/science/article/pii/S0769262585800581
2. https://pubmed.ncbi.nlm.nih.gov/14644097/

References