Difference between revisions of "Part:BBa K4477004:Design"
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===Source=== | ===Source=== | ||
| − | 1. https://www.sciencedirect.com/science/article/pii/S0769262585800581 | + | 1. https://www.sciencedirect.com/science/article/pii/S0769262585800581 [sequence of IK17]<br> |
| − | 2. https://pubmed.ncbi.nlm.nih.gov/14644097/ | + | 2. https://pubmed.ncbi.nlm.nih.gov/14644097/ [(G4S)3 sequence] |
===References=== | ===References=== | ||
Revision as of 07:58, 12 October 2022
McPC603 anti-oxLDL scFv antibody fragment
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 355
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 661
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Amino acid sequences reverse transcribed and codon optimized for expression in E. coli B (parent strain of SHuffle).
Source
1. https://www.sciencedirect.com/science/article/pii/S0769262585800581 [sequence of IK17]
2. https://pubmed.ncbi.nlm.nih.gov/14644097/ [(G4S)3 sequence]