Difference between revisions of "Part:BBa K4236100"
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The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS and UGT. F3H is an enzyme of 41.36 kD, which catalyzes naringenin into dihydrokaempferol. FLS is of 39.41 kD to catalyze dihydrokaempferol into kaempferol. We constructed an intact plasmid to express AtF3H and AtFLS respectively. | The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS and UGT. F3H is an enzyme of 41.36 kD, which catalyzes naringenin into dihydrokaempferol. FLS is of 39.41 kD to catalyze dihydrokaempferol into kaempferol. We constructed an intact plasmid to express AtF3H and AtFLS respectively. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 07:54, 12 October 2022
T7-RBS-atF3H+atFLS
The whole pathway in plants to produce astragalin contains three enzymes, F3H, FLS and UGT. F3H is an enzyme of 41.36 kD, which catalyzes naringenin into dihydrokaempferol. FLS is of 39.41 kD to catalyze dihydrokaempferol into kaempferol. We constructed an intact plasmid to express AtF3H and AtFLS respectively.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]