Difference between revisions of "Part:BBa K4488000"
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− | + | Salema, V., E. Marín, R. Martínez-Arteaga, D. Ruano-Gallego, S. Fraile, Y. Margolles, X. Teira, C. Gutierrez, G. Bodelón, and LÁ Fernández. 2013. 'Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies', PLoS One, 8: e75126. | |
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+ | Salema, V., and LÁ Fernández. 2017. 'Escherichia coli surface display for the selection of nanobodies', Microb Biotechnol, 10: 1468-84. | ||
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Revision as of 07:26, 12 October 2022
Neae: N terminus of 𝛃-intimin for Surface Display in E. coli
Intimin is an adhesion protein of EHEC. The N-terminus of beta-intimin (Neae) has been used for surface display in E. coli, specifically for sdAbs/Nanobodies. Neae consists of a trans-membrane beta barrel and requires the BAM complex for activation.
Reference: Salema, V., E. Marín, R. Martínez-Arteaga, D. Ruano-Gallego, S. Fraile, Y. Margolles, X. Teira, C. Gutierrez, G. Bodelón, and LÁ Fernández. 2013. 'Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies', PLoS One, 8: e75126.
Salema, V., and LÁ Fernández. 2017. 'Escherichia coli surface display for the selection of nanobodies', Microb Biotechnol, 10: 1468-84.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]