Difference between revisions of "Part:BBa K4156114"

Latest revision as of 07:19, 12 October 2022

pLldR-mRFP

pLldR-mRFP consists of the pLldR promoter ( BBa_K4156101 ) and a downstream RFP sequence. pLldR-mRFP was designed to test the performance of the pLldR promoter by detection of a red fluorescent signal.

Usage and Biology

In this BioBrick, we used the pLldR complex promoter to regulate the expression of the downstream mRFP and eventually introduced the rrnB T1 terminator. this design allowed the spatial and temporal intensity of gene expression under the pLldR promoter to be measured by the red fluorescent signal. We were thus able to verify the specific response of the pLldR promoter to lactate.

Characterization

lactate induced promoter testing

We constructed a lactate reporter consisting of the lactate-inducible promoter pLldR+mRFP. To test its performance, we added reporter in different chassis organisms.Fig 1 indicates that pLldR induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the lactate reporter can work properly.

control — **Figure2:**Induction of downstream gene mRFP expression over time by the AR reporter consisting of plldR+mRFP at different lactate concentrations.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 680
Illegal AgeI site found at 1908
Illegal AgeI site found at 2020
1000
COMPATIBLE WITH RFC[1000]

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 ==lactate induced promoter testing==
-We constructed a lactate  reporter consisting of the lactate-inducible promoter pPepT+mRFP. To test its performance, we added reporter in different chassis organisms.Fig 1 indicates that pLldR induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the lactate reporter can work properly.
+We constructed a lactate  reporter consisting of the lactate-inducible promoter pLldR+mRFP. To test its performance, we added reporter in different chassis organisms.Fig 1 indicates that pLldR induces the expression of the downstream gene mRFP with the decrease of O2. Thus, it can be seen that the lactate reporter can work properly.
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