Difference between revisions of "Part:BBa K118011"

 
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This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
 
This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.)
  
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===Usage and Biology===
 
===Usage and Biology===
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=ShanghaiTech_China 2022's Characterization=
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[[File:Results-PcstA&PcsiE with LVA.png|600px|thumb|center|alt=domestication.|Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K118011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K118011 SequenceAndFeatures</partinfo>

Revision as of 07:14, 12 October 2022


PcstA (glucose-repressible promoter)

This is the promoter for the Eschaerichia coli JM109 cstA gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (cstA encodes the carbon starvation protein.)


Usage and Biology

ShanghaiTech_China 2022's Characterization

domestication.
Figrure 1.The result of sfGFP (with LVA) expression under the conditions of different carbon source concentrations

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]