Difference between revisions of "Part:BBa K4229067"
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It was later confirmed that in fact mTurqouise2 was cached by T1 by a western blot comparing the full wiffleball and the minimal wiffleball. | It was later confirmed that in fact mTurqouise2 was cached by T1 by a western blot comparing the full wiffleball and the minimal wiffleball. | ||
− | [[File: MTurquoiseWestern.png|800px|thumb|left|: Western Blot of the BMC minimal wiffleball (pT1spysnp) and full wiffleball (pT1spysnpT2T3) + mTurquoise2]] | + | [[File:MTurquoiseWestern.png|800px|thumb|left|: Western Blot of the BMC minimal wiffleball (pT1spysnp) and full wiffleball (pT1spysnpT2T3) + mTurquoise2]] |
Revision as of 07:13, 12 October 2022
mTurquoise2 with C-terminal SnoopCatcher regulated by tetA/B promotor
mTurqouise2 is with mVenus2(BBa_K4229066) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078).
The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see a fluorescent signal in the bacteria that expressed either of those two. You can see foci (especially well with the full wiffleball) for both of those reporter genes:
It was later confirmed that in fact mTurqouise2 was cached by T1 by a western blot comparing the full wiffleball and the minimal wiffleball.
With that, we can say that our mTurquiose2 with the snoopCatcher is successfully expressed and caught by our compartment.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
Illegal PstI site found at 856 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
Illegal PstI site found at 856 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BglII site found at 68 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
Illegal PstI site found at 856 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
Illegal PstI site found at 856
Illegal AgeI site found at 121
Illegal AgeI site found at 1017 - 1000COMPATIBLE WITH RFC[1000]