Difference between revisions of "Part:BBa K4229067"

Revision as of 07:12, 12 October 2022

mTurquoise2 with C-terminal SnoopCatcher regulated by tetA/B promotor

mTurqouise2 is with mVenus2(BBa_K4229066) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078).

The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see a fluorescent signal in the bacteria that expressed either of those two. You can see foci (especially well with the full wiffleball) for both of those reporter genes:

Fluorescent microscopy of T1 catching the mVenus2 and mturquoise2 when the minimal or full BMC construct is expressed: A Controls for induction; B T1 with and without the Spy/Snp tags; scalebar 5µm

It was later confirmed that in fact mTurqouise2 was cached by T1 by a western blot comparing the full wiffleball and the minimal wiffleball. [[File: MTurquoiseWestern.png|800px|thumb|left|: Western Blot of the BMC minimal wiffleball (pT1spysnp) and full wiffleball (pT1spysnpT2T3) + mTurquoise2]]

With that, we can say that our mTurquiose2 with the snoopCatcher is successfully expressed and caught by our compartment.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 59
Illegal PstI site found at 856
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 59
Illegal PstI site found at 856
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 59
Illegal BglII site found at 68
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 59
Illegal PstI site found at 856
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 59
Illegal PstI site found at 856
Illegal AgeI site found at 121
Illegal AgeI site found at 1017
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4229067 short</partinfo>
-mTurquoise2 fused with snoopCatcher C-terminal regulated by tetA/B promotor
+mTurqouise2 is with mVenus2(BBa_K4229066) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078).
+The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see a fluorescent signal in the bacteria that expressed either of those two. You can see foci (especially well with the full wiffleball) for both of those reporter genes:
+[[File:MVenusmTurquoise.png|800px|thumb|left|Fluorescent microscopy of T1 catching the mVenus2 and mturquoise2 when the minimal or full BMC construct is expressed: A Controls for induction; B T1 with and without the Spy/Snp tags; scalebar 5µm]]
+It was later confirmed that in fact mTurqouise2 was cached by T1 by a western blot comparing the full wiffleball and the minimal wiffleball.
+[[File:	MTurquoiseWestern.png|800px|thumb|left|: Western Blot of the BMC minimal wiffleball (pT1spysnp) and full wiffleball (pT1spysnpT2T3) + mTurquoise2]]
+With that, we can say that our mTurquiose2 with the snoopCatcher is successfully expressed and caught by our compartment.
 <!-- Add more about the biology of this part here