Difference between revisions of "Part:BBa K4117666:Experience"

Revision as of 07:11, 12 October 2022

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Applications of BBa_K4117666

We added the GFP downstream of the RNA thermometer (RBS) to characterize the efficiency of RNA thermometer.

Fig.1 RNA thermometer verification pathway

In order to verify the function of RNA thermometers, We cultured the strain at 37°C overnight, and then aliquots, adding pre-prepared flasks filled with 37°C and 28°C LB medium to make the final volume 20ml, then measured the initial OD600 and GFP fluorescence intensity. Samples were taken once every half-hour, OD600 and GFP fluorescence intensity were measured.

GFP measurement results are shown in Fig.2.

[[File:|500px]] Fig.2 GFP fluorescence intensity after incubating in different temperatures.

User Reviews

UNIQ739de9d27d2e1dec-partinfo-00000000-QINU UNIQ739de9d27d2e1dec-partinfo-00000001-QINU

@@ Line 6: / Line 6: @@
 We added the GFP downstream of the RNA thermometer (RBS) to characterize the efficiency of RNA thermometer.<br>
-<center>[[File:T--BNU-China--GFPpathway.png|500px]]</center><br>
+<center>[[File:T--BNU-China--GFPpathway.png|500px]]</center>
 <center>Fig.1 RNA thermometer verification pathway</center><br>
+In order to verify the function of RNA thermometers, We cultured the strain at 37°C overnight, and then aliquots, adding pre-prepared flasks filled with 37°C and 28°C LB medium to make the final volume 20ml, then measured the initial OD600 and GFP fluorescence intensity. Samples were taken once every half-hour, OD600 and GFP fluorescence intensity were measured.<br>
+GFP measurement results are shown in '''Fig.2'''.<br>
+<center>[[File:|500px]]</center>
+<center>Fig.2 GFP fluorescence intensity after incubating in different temperatures.</center><br>
 ===User Reviews===