Difference between revisions of "Part:BBa K4229066"

Revision as of 06:53, 12 October 2022

mVenus with N-terminal spyCatcher regulated by tetA/B promotor

mVenus2 is with mTurqouise2(BBa_K4229067) one of our reporter genes, with which we tested the building and catching of the minimal(BBa_K4229047) and full wiffleball(BBa_K4229049) and SPD5 (BBa_K4229078).

The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see an flourecent signal in the bacteria which expressed wither of those two:

Fluorescent microscopy of T1 catching the mVenus2 and mturquoise2 when the minimal or full BMC construct is expressed: A Controls for induction; B T1 with and without the Spy/Snp tags; scalebar 5µm

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 59
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 59
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 59
Illegal BglII site found at 68
Illegal XhoI site found at 1075
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 59
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 59
Illegal AgeI site found at 508
1000
COMPATIBLE WITH RFC[1000]

Revision as of 06:52, 12 October 2022 (view source) NikitaEdel (Talk \| contribs) ← Older edit		Revision as of 06:53, 12 October 2022 (view source) NikitaEdel (Talk \| contribs) Newer edit →
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	The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see an flourecent signal in the bacteria which expressed wither of those two:		The snoop/spyTag snoop/spyCatcher was tested with those two reporter genes. You can see an flourecent signal in the bacteria which expressed wither of those two:

−	[[File:MVenusmTurquoise.png\|~~900px~~\|thumb\|left\|Fluorescent microscopy of T1 catching the mVenus2 and mturquoise2 when the minimal or full BMC construct is expressed: A Controls for induction; B T1 with and without the Spy/Snp tags; scalebar 5µm]]	+	[[File:MVenusmTurquoise.png\|800px\|thumb\|left\|Fluorescent microscopy of T1 catching the mVenus2 and mturquoise2 when the minimal or full BMC construct is expressed: A Controls for induction; B T1 with and without the Spy/Snp tags; scalebar 5µm]]