Difference between revisions of "Part:BBa K4274011"
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==Sequence and Features== | ==Sequence and Features== | ||
| − | <partinfo> | + | <partinfo>BBa_K4274011 SequenceAndFeatures</partinfo> |
==References== | ==References== | ||
[1]Nambiar T., Billon P., Diedenhofen G., et al. Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nature Communications. 10 (5), 2295 (2019). https://doi.org/10.1038/s41467-019-11105-z | [1]Nambiar T., Billon P., Diedenhofen G., et al. Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nature Communications. 10 (5), 2295 (2019). https://doi.org/10.1038/s41467-019-11105-z | ||
Latest revision as of 06:37, 12 October 2022
sfp_HA_US
sfp_HA_US is a homologous donor template situated in the upstream of sfp gene of Bacillus subtilis. After knock-out the natural sfp gene, sfp_HA_US could stimulate the homology-directed repair(HDR) of site-specific DNA double-strand breaks (DSBs) induced by the RNA-guided Cas9 endonuclease. Homology-directed repair (HDR) of these DSBs enables precise editing of the genome by introducing defined genomic changes, including base substitutions, sequence insertions, and deletions. It was used in the composite part PvanP*-sfp_target-sfp_HA_US-p43-K4274013-sfp-K4274014-degQ-sfp_HA_DS (Part: BBa_K4274035) to realize fengycins’ production in B. subtilis.
Source
Bacillus subtilis 168
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1048
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1048
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1048
- 1000COMPATIBLE WITH RFC[1000]
References
[1]Nambiar T., Billon P., Diedenhofen G., et al. Stimulation of CRISPR-mediated homology-directed repair by an engineered RAD18 variant. Nature Communications. 10 (5), 2295 (2019). https://doi.org/10.1038/s41467-019-11105-z