Difference between revisions of "Part:BBa K4509420:Design"
(→Source and Improvement) |
(→Source and Improvement) |
||
| Line 8: | Line 8: | ||
===Source and Improvement=== | ===Source and Improvement=== | ||
| − | '''Source:''' Part:[ | + | '''Source:''' Part:[https://parts.igem.org/Part:BBa_K1291071 BBa_K1291071] |
HORSERADISH PEROXIDASE TYPE C COMPOSITE PART. | HORSERADISH PEROXIDASE TYPE C COMPOSITE PART. | ||
Revision as of 06:12, 12 October 2022
HORSERADISH PEROXIDASE with constitutive promoter J23116
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 445
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 565
Illegal AgeI site found at 719
Illegal AgeI site found at 1022 - 1000COMPATIBLE WITH RFC[1000]
Source and Improvement
Source: Part:BBa_K1291071 HORSERADISH PEROXIDASE TYPE C COMPOSITE PART.
The promoter J23119 is replaced with J23116. This switch in the design will reduce the burden on the cell during the overexpression of enzymes.