Difference between revisions of "Part:BBa K4438173"

 
Line 5: Line 5:
 
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This is the expression cassette for BBa_K4438113 with BBa_J64997 upstream. FASTmiR-27a-D1_p, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438502. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
 
FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This is the expression cassette for BBa_K4438113 with BBa_J64997 upstream. FASTmiR-27a-D1_p, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438502. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.
  
−
<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
 +
This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given:
  
 
<!-- -->
 
<!-- -->
Line 17: Line 17:
 
<partinfo>BBa_K4438173 parameters</partinfo>
 
<partinfo>BBa_K4438173 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===Notes===
 +
The split position of the complementary sequence in the sensor is TGCTCACAAGCAGC….TAAGCCCT
 +
 +
===References===
 +
Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130

Revision as of 05:55, 12 October 2022


FASTmiR-27a-D1_p

FASTmiR stands for Fluorescence Aptamer Sensor For Tracking miRNAs. It is an RNA-based sensor for in-vitro quantification of miRNAs. This is the expression cassette for BBa_K4438113 with BBa_J64997 upstream. FASTmiR-27a-D1_p, after transcription, binds to miR-27a-5p, the transcription product of BBa_K4438502. The binding of these to RNAs will lead to the revelation of a binding site for DFHBI, a fluorophore, where it gets trapped and shows fluorescence.

Usage and Biology

This part can be used for the detection of miR-27a-5p, which proves to be a biomarker for many diseases. The promoter sequence, in the beginning, enhances its usage in both in-vivo and in-vitro conditions. The ON and OFF structure for the sensor was generated as given:

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 72
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 72
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 72
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 72
  • 1000
    COMPATIBLE WITH RFC[1000]


Notes

The split position of the complementary sequence in the sensor is TGCTCACAAGCAGC….TAAGCCCT

References

Huang, K., Doyle, F., Wurz, Z. E., Tenenbaum, S. A., Hammond, R. K., Caplan, J. L., & Meyers, B. C. (2017). FASTmiR: an RNA-based sensor for in vitro quantification and live-cell localization of small RNAs. Nucleic acids research, 45(14), e130-e130