Difference between revisions of "Part:BBa K4204004"
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This part is the whole gene circuit of EilR, the repressor of the crystal violet induction system. This sequence contains a promoter, RBS, and the coding sequence of eilR, and a strong terminator. | This part is the whole gene circuit of EilR, the repressor of the crystal violet induction system. This sequence contains a promoter, RBS, and the coding sequence of eilR, and a strong terminator. | ||
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| + | For detailed characterization,see BBa_K4204016 | ||
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Revision as of 05:01, 12 October 2022
Transcriptional unit of EilR.
Crystal violet induction system is a cheap, efficient and sensitive induction system. The repressor protein EilR could bind to operator eilOc and inhibit transcription when crystal violet is absent. Its working principle is shown in the figure below.
This part is the whole gene circuit of EilR, the repressor of the crystal violet induction system. This sequence contains a promoter, RBS, and the coding sequence of eilR, and a strong terminator.
For detailed characterization,see BBa_K4204016
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 475
Illegal AgeI site found at 625 - 1000COMPATIBLE WITH RFC[1000]