Difference between revisions of "Part:BBa K4192142:Design"
 
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===Design Notes===
 
===Design Notes===
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The construction was unsuccessful, and PcysH was skipped directly in gibson assembly to construct PcysJ-mazE-mazF, probably due to the homologous arm design
+
This element showed no inducibility in Pseudomonas fluorescein and resulted in 85% bacterial death, probably due to essential Escherichia coli regulatory elements.
−
 
+
 
===Source===
 
===Source===
 
All individual components were derived from E. coli.
 
All individual components were derived from E. coli.

Latest revision as of 04:50, 12 October 2022


PcysJ-mazE-mazF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This element showed no inducibility in Pseudomonas fluorescein and resulted in 85% bacterial death, probably due to essential Escherichia coli regulatory elements.

Source

All individual components were derived from E. coli.

References

[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1).