Difference between revisions of "Part:BBa K4444004"

 
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<partinfo>BBa_K4444004 short</partinfo>
 
<partinfo>BBa_K4444004 short</partinfo>
  
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This is a modified backbone from the RELEASE plamid. It does not contain a furin cut site. This backbone contains GFP, a tri-transmembrane domain, and ER retention motif. The plasmid uses ampicillin resistance.
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PhoCl Display mRuby Backbone is modified from the RELEASE system. It contains a GFP as the protein of interest, PhoCl2c, a tri-transmembrane domain and an ER retention motif. The furin cut site and HCV cut site were removed. PhoCl Display will display a protein of interest on the cell membrane in response to ultraviolet light stimulation.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:31, 12 October 2022


PhoCl Display GFP

PhoCl Display mRuby Backbone is modified from the RELEASE system. It contains a GFP as the protein of interest, PhoCl2c, a tri-transmembrane domain and an ER retention motif. The furin cut site and HCV cut site were removed. PhoCl Display will display a protein of interest on the cell membrane in response to ultraviolet light stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 898
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1021
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1543