Difference between revisions of "Part:BBa K4444003"

 
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<partinfo>BBa_K4444003 short</partinfo>
 
<partinfo>BBa_K4444003 short</partinfo>
  
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This backbone contains the plasmid encoding RELEASE, with the furin cut site, HCV cut site, and ER retention motif removed. We inserted PhoCl into the previous furin cut site location. The plasmid contains ampicillin resistance.
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PhoCl Shed mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, PhoCl2c, and a tri-transmembrane domain; the ER retention motif, furin cut site, and HCV cut site were all removed. PhoCl Shed will keep the protein of interest on the cell membrane until violet light is shined on it, which then breaks PhoCl and releases all the protein from the surface of the cell membrane.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 04:26, 12 October 2022


PhoCl Shed GFP

PhoCl Shed mRuby is modified from the RELEASE system. It contains a GFP as a protein of interest, PhoCl2c, and a tri-transmembrane domain; the ER retention motif, furin cut site, and HCV cut site were all removed. PhoCl Shed will keep the protein of interest on the cell membrane until violet light is shined on it, which then breaks PhoCl and releases all the protein from the surface of the cell membrane.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 60
    Illegal BsaI.rc site found at 1294