Difference between revisions of "Part:BBa K4193027:Experience"
| Line 13: | Line 13: | ||
<body> | <body> | ||
<div> | <div> | ||
| − | <img src=""> | + | <img src="https://static.igem.wiki/teams/4193/wiki/other/part/ssre-expression.jpg"> |
</div> | </div> | ||
</body> | </body> | ||
| Line 27: | Line 27: | ||
<body> | <body> | ||
<div> | <div> | ||
| − | <img src=""> | + | <img src="https://static.igem.wiki/teams/4193/wiki/other/part/phophoylated-function-protein-changes.jpg"> |
</div> | </div> | ||
</body> | </body> | ||
Latest revision as of 04:23, 12 October 2022
IP concentration gradient was set to cultivate Engineered microorganism. When IP accumulates to a certain extent in the environment, it can bind with AtCRE1 to phosphorylate downstream Ypd1, and transmit phosphate signal to Skn7, so that Skn7 can induce the expression of SSRE promoter
Applications of BBa_K4193027
Different concentrations of IP can increase the expression of SSRE promoter. After rough statistical analysis, the expression intensity of SSRE promoter was significantly correlated with IP concentration.
To get a great insight for the protein in designed signal pathway, we used dry lab to make up for the defect we could not characterize the behavior of these proteins, such as AtCRE1_P, Ypd1_P and Skn7_P interacting with different subtypes of SSRE promoter.
User Reviews
UNIQ2af44812fae902eb-partinfo-00000002-QINU UNIQ2af44812fae902eb-partinfo-00000003-QINU