Difference between revisions of "Part:BBa K4444015"

 
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<partinfo>BBa_K4444015 short</partinfo>
 
<partinfo>BBa_K4444015 short</partinfo>
  
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Renillla Luciferase comes from a firefly and is a protein that produces fluorescence when it oxidizes its substrate. We assayed the media containing the luciferase using the Promega Luciferase Assay System to analyze the fluorescence of the media.
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PhoCl Secrete Luciferase is modified from the RELEASE system. It contains a luciferase as a protein of interest, a furin cut site, PhoCl2c, tri-transmembrane domain, and an ER retention motif. PhoCl Secrete will completely release a protein of interest in response to ultra violet light stimulation.
  
 
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Revision as of 04:02, 12 October 2022


PhoCl Secrete Luciferase

PhoCl Secrete Luciferase is modified from the RELEASE system. It contains a luciferase as a protein of interest, a furin cut site, PhoCl2c, tri-transmembrane domain, and an ER retention motif. PhoCl Secrete will completely release a protein of interest in response to ultra violet light stimulation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 991
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 604
    Illegal BsaI.rc site found at 1684