Difference between revisions of "Part:BBa K4169030"
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===Usage and Biology===
 
===Usage and Biology===
 
When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.
 
When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.
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===Functional Parameters===
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<partinfo>BBa_K4169030 parameters</partinfo>
 
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===Sequence and Features===
 
===Sequence and Features===
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===Functional Parameters===
 
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<partinfo>BBa_K4169030 parameters</partinfo>
 
 
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Revision as of 03:54, 12 October 2022


Cre-reverse loxP system:working when you need to express genes in the other direction

In the presence of theophylline, expression of subsequent Cre recombinase is induced.In addition, the promoter and RBS between the two loxP will be reversed, making it express genes in the other direction.

Usage and Biology

When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.

Functional Parameters

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1292
    Illegal NheI site found at 1315
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 39
    Illegal AgeI site found at 140
  • 1000
    COMPATIBLE WITH RFC[1000]