Difference between revisions of "Part:BBa K4322004"
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+ | <partinfo>BBa_K4322004 short</partinfo> | ||
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+ | When csgA-alpha fusion chromoproteins are expressed in a culture of E. coli which is then co-cultured with cells secreting csgA-gamma(BBa K4322006), polymers of csgA-alpha-chromoprotein and csgA-gamma will form. The fibrin knob domain/alpha (BBa K4322001) and the fibrin hole domain/gamma (BBa K4322002) will interact (as they do in fibrin [1]) to form long polymers that are covalently linked to chromoproteins [2]. This part is a fusion of the csgA-alpha fusion protein (BBa_K4322005), a glycine-serine linker sequence, and the amajLime chromoprotein (BBa_K1033914). | ||
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+ | ==References:== | ||
+ | [1]R. I. Litvinov et al., “Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions,” Blood, vol. 109, no. 1, pp. 130–138, Jan. 2007, doi: 10.1182/blood-2006-07-033910. | ||
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+ | [2]A. M. Duraj-Thatte et al., “Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers,” Nat Commun, vol. 12, no. 1, p. 6600, Dec. 2021, doi: 10.1038/s41467-021-26791-x. | ||
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+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K4322004 SequenceAndFeatures</partinfo> | ||
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+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K4322004 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 03:52, 12 October 2022
csgAα-amajLime fusion protein with glycine-serine linker
When csgA-alpha fusion chromoproteins are expressed in a culture of E. coli which is then co-cultured with cells secreting csgA-gamma(BBa K4322006), polymers of csgA-alpha-chromoprotein and csgA-gamma will form. The fibrin knob domain/alpha (BBa K4322001) and the fibrin hole domain/gamma (BBa K4322002) will interact (as they do in fibrin [1]) to form long polymers that are covalently linked to chromoproteins [2]. This part is a fusion of the csgA-alpha fusion protein (BBa_K4322005), a glycine-serine linker sequence, and the amajLime chromoprotein (BBa_K1033914).
References:
[1]R. I. Litvinov et al., “Polymerization of fibrin: direct observation and quantification of individual B:b knob-hole interactions,” Blood, vol. 109, no. 1, pp. 130–138, Jan. 2007, doi: 10.1182/blood-2006-07-033910.
[2]A. M. Duraj-Thatte et al., “Programmable microbial ink for 3D printing of living materials produced from genetically engineered protein nanofibers,” Nat Commun, vol. 12, no. 1, p. 6600, Dec. 2021, doi: 10.1038/s41467-021-26791-x.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1128
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 58
- 1000COMPATIBLE WITH RFC[1000]