Difference between revisions of "Part:BBa K4509420"
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The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23116 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site.
 
The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23116 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4509420 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4509420 parameters</partinfo>
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==Characterization==

Revision as of 03:10, 12 October 2022


HORSERADISH PEROXIDASE with constitutive promoter J23116

The enzyme horseradish peroxidase, found in the roots of horseradish catalyzes the oxidation of various organic substrates by hydrogen peroxide. It is a metalloenzyme with many isoforms and the most studied type is C. The composite part starts with the J23116 constitutive promoter, continues with RBS and is followed by the Horseradish Peroxidase coding sequence. The sequence ends with a terminator and a BamH1 restriction site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 445
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 565
    Illegal AgeI site found at 719
    Illegal AgeI site found at 1022
  • 1000
    COMPATIBLE WITH RFC[1000]



Characterization