Difference between revisions of "Part:BBa K4440000"
Kiselev sl (Talk | contribs) |
Kiselev sl (Talk | contribs) (→Usage and Biology) |
||
| Line 16: | Line 16: | ||
[[Image:Vp2c part.svg|600px|thumb|center|<b>Figure 2.</b> Observable color change in VP2C-Tyr1 expressing yeast. ]] | [[Image:Vp2c part.svg|600px|thumb|center|<b>Figure 2.</b> Observable color change in VP2C-Tyr1 expressing yeast. ]] | ||
| + | [[Image:Vp2c flu part.png|800px|thumb|center<b>Figure 3.</b> Microscopy images demonstrating an increase in cell fluorescence upon VP2C-Tyr1 expression, indicative of the presence of some pigment.]] | ||
| + | |||
| + | |||
| − | |||
<!-- --> | <!-- --> | ||
Revision as of 03:02, 12 October 2022
VP2C-Tyr1
VP2C-Tyr1 fusion protein
Usage and Biology
This part was created for a strategy in which we aimed to concentrate the tyrosinase (Tyr1[BBa_K4440002]) and melanin production into virus-like nanoparticles with the aim to minimize toxicity. For this, the cells expressed viral VP1 protein and the tyrosinase was expressed as a fusion to viral VP2C protein (Figure 1). In this way, VP1 and VP2C multimerize to form a particle, where tyrosinase was localized in the particle.
In order to produce melanin in nanocompartments this sequence was put under GAL1/10 promoter to produce both parts of the compartment simultaneously.
We tested the effect of this part in yeast with several experiments: visually the yeast changed color because of a produced pigment (Figure 2), increased cellular fluorescence upon expression of VP2C-Tyr1 was observed (Figure 3).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]