Difference between revisions of "Part:BBa K4166007"
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Preventing R loop formation | Preventing R loop formation | ||
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| + | == Plasmid Copy Number and Expression - Alma 2022 == | ||
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| + | We created a series of mutants of BBa_J04450 in the origin of replication, and wanted to both quantify the copy number and see the effect the copy number has on gene expression. | ||
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| + | [[File:T--Alma--pcnPlate.png|700px|center]] | ||
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| + | According to some gel quantitation of plasmid copy number, we estimate that this strain has a copy number of 197 plasmids per cell. This may explain the slight decrease in expression. | ||
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Latest revision as of 03:00, 12 October 2022
Camps -11
Preventing R loop formation
Plasmid Copy Number and Expression - Alma 2022
We created a series of mutants of BBa_J04450 in the origin of replication, and wanted to both quantify the copy number and see the effect the copy number has on gene expression.
According to some gel quantitation of plasmid copy number, we estimate that this strain has a copy number of 197 plasmids per cell. This may explain the slight decrease in expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.