Difference between revisions of "Part:BBa K4390103"

 
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<partinfo>BBa_K4390103 short</partinfo>
 
<partinfo>BBa_K4390103 short</partinfo>
  
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==Improvement of part BBa_K3380600==
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This part is an improvement of [[Part:BBa_K3380600]], as we have swapped the T7 promoter to one no longer containing a BsaI site. This means it can be used in Type IIS assembly techniques such as JUMP assembly, which was our primary assembly technique for the iGEM project.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:58, 12 October 2022


arsR controlled iSpinach biosensor

Improvement of part BBa_K3380600

This part is an improvement of Part:BBa_K3380600, as we have swapped the T7 promoter to one no longer containing a BsaI site. This means it can be used in Type IIS assembly techniques such as JUMP assembly, which was our primary assembly technique for the iGEM project.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 52
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 52
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]