Difference between revisions of "Part:BBa K4390105"
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<partinfo>BBa_K4390105 short</partinfo> | <partinfo>BBa_K4390105 short</partinfo> | ||
| − | - | + | ==Improvement of part BBa_K3380600== |
| + | This part is an improvement of [[Part:BBa_K3380600]], as it uses a brigther aptamer. We designed a TXO ArsR-regulated T7 promoter biosensor expressing Broccoli instead of iSpinach ([[Part:BBa_K4390105]]). Broccoli binds DFHBI more tightly than iSpinach, hence producing brighter fluorescence (See Figure 1). | ||
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| + | <br> | ||
| + | [[File:Various aptamers.png|800px]] | ||
| + | <br> | ||
| + | Figure 1: The brightness of various aptamers in combination with various fluorophores. The column labelled "Green" represents iSpinach. | ||
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Revision as of 02:56, 12 October 2022
arsR controlled Broccoli biosensor
Improvement of part BBa_K3380600
This part is an improvement of Part:BBa_K3380600, as it uses a brigther aptamer. We designed a TXO ArsR-regulated T7 promoter biosensor expressing Broccoli instead of iSpinach (Part:BBa_K4390105). Broccoli binds DFHBI more tightly than iSpinach, hence producing brighter fluorescence (See Figure 1).
Figure 1: The brightness of various aptamers in combination with various fluorophores. The column labelled "Green" represents iSpinach.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 52
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 52
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 52
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 52
- 1000COMPATIBLE WITH RFC[1000]