Difference between revisions of "Part:BBa K4169030"

Revision as of 02:50, 12 October 2022

Cre-reverse loxP system:working when you need to express genes in the other direction

In the presence of theophylline, expression of subsequent Cre recombinase is induced.In addition, the promoter and RBS between the two loxP will be reversed, making it express genes in the other direction.

Usage and Biology

When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1292
Illegal NheI site found at 1315
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 454
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 39
Illegal AgeI site found at 140
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.<!-- -->
+When our engineered bacteria feel theophylline, Cre expresses it, Cre recognizes the reverse repeat sequence at both ends of the loxP site and combines to form a dimer, which then binds to the dimer on another loxP site to form a tetramer. This way Cre mediates the reverse sequence reversal between loxPs.
+<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4169030 SequenceAndFeatures</partinfo>