Difference between revisions of "Part:BBa K4229044:Design"

 
 
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===Source===
 
===Source===
  
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Was kindly send to us by the Kerfeld group. From this paper https://doi.org/10.1073/pnas.2116871119
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Was kindly send to us by the Kerfeld group.  
  
 
===References===
 
===References===
 +
 +
[1] C. A. Kerfeld, C. Aussignargues, J. Zarzycki, F. Cai, and M. Sutter, “Bacterial microcompartments,” Nat. Rev. Microbiol., vol. 16, no. 5, pp. 277–290, 2018, doi: 10.1038/nrmicro.2018.10.
 +
 +
[2] H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.

Latest revision as of 02:37, 12 October 2022


H,T1,T2,T3 assemble the "full wiffelball"


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1061
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1061
    Illegal NotI site found at 865
    Illegal NotI site found at 2242
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1061
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1061
    Illegal NgoMIV site found at 384
    Illegal NgoMIV site found at 1862
    Illegal NgoMIV site found at 2218
    Illegal AgeI site found at 669
    Illegal AgeI site found at 777
    Illegal AgeI site found at 1364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Was kindly send to us by the Kerfeld group.

References

[1] C. A. Kerfeld, C. Aussignargues, J. Zarzycki, F. Cai, and M. Sutter, “Bacterial microcompartments,” Nat. Rev. Microbiol., vol. 16, no. 5, pp. 277–290, 2018, doi: 10.1038/nrmicro.2018.10.

[2] H. Kirst, B. H. Ferlez, S. N. Lindner, C. A. R. Cotton, A. Bar-Even, and C. A. Kerfeld, “Toward a glycyl radical enzyme containing synthetic bacterial microcompartment to produce pyruvate from formate and acetate,” Proc. Natl. Acad. Sci. U. S. A., vol. 119, no. 8, pp. 1–10, 2022, doi: 10.1073/pnas.2116871119.