Difference between revisions of "Part:BBa K4472994:Design"

 
 
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===Source===
 
===Source===
  
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The guide RNA is complementary to the endogenous E.coli mRNA hcat.
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The guide RNA is complementary to the stable endogenous E.coli mRNA hcat.
  
 
===References===
 
===References===
 +
 +
Zhou K, Zhou L, Lim Q, Zou R, Stephanopoulos G, Too HP. Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR. BMC Mol Biol. 2011;12(1):1-9. doi:10.1186/1471-2199-12-18/FIGURES/5
 +
 +
https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?from=2666707&to=2667846&report=genbank&strand=true

Latest revision as of 02:36, 12 October 2022


guideRNA 1 detecting hcat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The guideRNA should have a length of 82 basepairs (Gambill L, Staubus A, Ameruoso A, Chappell J. A split ribozyme that links detection of a native RNA to orthogonal protein outputs. bioRxiv. January 2022:2022.01.12.476080. doi:10.1101/2022.01.12.476080)


Source

The guide RNA is complementary to the stable endogenous E.coli mRNA hcat.

References

Zhou K, Zhou L, Lim Q, Zou R, Stephanopoulos G, Too HP. Novel reference genes for quantifying transcriptional responses of Escherichia coli to protein overexpression by quantitative PCR. BMC Mol Biol. 2011;12(1):1-9. doi:10.1186/1471-2199-12-18/FIGURES/5

https://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?from=2666707&to=2667846&report=genbank&strand=true