Difference between revisions of "Part:BBa K4201014"

 
 
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<partinfo>BBa_K4201014 short</partinfo>
 
<partinfo>BBa_K4201014 short</partinfo>
  
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This Chloramphenicol Backbone conveys antibiotic resistance in transformed bacteria. The backbone also contains a Blue/White marker which is flanked by PaqCI and BsaI cut sites. This marker is excised during Golden Gate gene assembly and replaced by a gene of interest, allowing blue/white selection of transformed colonies. This backbone was used as an intermediate to be transformed into E. coli, before a gene of interest was inserted into an agrobacterium-ready pLSUK backbone.  
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This Chloramphenicol Backbone conveys antibiotic resistance in transformed bacteria. The backbone also contains a Blue/White marker which is flanked by PaqCI and BsaI cut sites. This marker is excised during Golden Gate gene assembly and replaced by a gene of interest, allowing blue/white selection of transformed colonies. This backbone was used as an intermediate to be transformed into <i>E. coli</i>, before a gene of interest was inserted into an agrobacterium-ready pLSUK backbone.  
  
  

Latest revision as of 02:33, 12 October 2022


Chlor AarI

This Chloramphenicol Backbone conveys antibiotic resistance in transformed bacteria. The backbone also contains a Blue/White marker which is flanked by PaqCI and BsaI cut sites. This marker is excised during Golden Gate gene assembly and replaced by a gene of interest, allowing blue/white selection of transformed colonies. This backbone was used as an intermediate to be transformed into E. coli, before a gene of interest was inserted into an agrobacterium-ready pLSUK backbone.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 320
    Illegal XbaI site found at 293
    Illegal PstI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 14
    Illegal BsaI.rc site found at 736