Difference between revisions of "Part:BBa K4201009"

Latest revision as of 02:17, 12 October 2022

Arabidopsis thaliana Heat Shock Protein (AtHSP)

AtHSP is a terminator that was discovered from the analysis of the A. thaliana genome¹. It contains poly A regions where the mRNA is cleaved, which helps increase the expression of the desired transgene¹. When compared to other A. thaliana terminators, AtHSP was effective in increasing expression levels of the desired transgene^1,2. Heat shock proteins (HSPs) are involved in many different essential cellular processes, which makes AtHSP a good candidate for increasing the expression of a transgene³. Additional benefits of using AtHSP include its small size, which helps reduce the overall size of the desired genetic construct.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 184
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 184
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 184
Illegal BglII site found at 800
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 184
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 184
Illegal NgoMIV site found at 333
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1517

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4201009 short</partinfo>
-AtHSP is a terminator that was discovered from the analysis of the <i>A. thaliana genome</i><sup>1</sup>. It contains poly A regions where the mRNA is cleaved, which helps increase the expression of the desired transgene<sup>1</sup>.  When compared to other <i>A. thaliana </i>terminators, AtHSP was effective in increasing expression levels of the desired transgene<sup>1,2</sup>.
+AtHSP is a terminator that was discovered from the analysis of the <i>A. thaliana</i> genome<sup>1</sup>. It contains poly A regions where the mRNA is cleaved, which helps increase the expression of the desired transgene<sup>1</sup>.  When compared to other <i>A. thaliana </i>terminators, AtHSP was effective in increasing expression levels of the desired transgene<sup>1,2</sup>.
 Heat shock proteins (HSPs) are involved in many different essential cellular processes, which makes AtHSP a good candidate for increasing the expression of a transgene<sup>3</sup>.  Additional benefits of using AtHSP include its small size, which helps reduce the overall size of the desired genetic construct.