Difference between revisions of "Part:BBa K4156118"
(→‎Characterization)
 
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Characterization details can be found at：
 
Characterization details can be found at：
  
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pCadC-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>  
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pCadC-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156099"> BBa_K4156099 </a></html>  
  
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pCadC-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>  
+
pCadC-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156100"> BBa_K4156100 </a></html>  
  
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pLldR-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>  
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pLldR-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156104"> BBa_K4156104 </a></html>  
  
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pLldR-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>  
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pLldR-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156105"> BBa_K4156105 </a></html>  
  
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pPepT-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>  
+
pPepT-TP901:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156108"> BBa_K4156108 </a></html>
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 +
pPepT-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156109"> BBa_K4156109 </a></html>  
  
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pPepT-TP901-φ174E:<html><a style="padding: 0px; margin: 0px;" href="https://parts.igem.org/Part:BBa_K4156112"> BBa_K4156112 </a></html>
 
  
  

Latest revision as of 02:04, 12 October 2022


XOR gate-mRFP

XOR gate-mRFP consists of an XOR logic gate ( BBa_K4156117 ) and a downstream RFP sequence to test the performance of the XOR gate by detecting the red fluorescent signal.


Usage and Biology

We introduced a dual vector system in the same strain. The XOR gate-mRFP is located on pSB4C5, while its upstream promoter and corresponding TP901/BxB1 integrase are located on another plasmid, pSB1A3, which are resistant to chloramphenicol and ampicillin, respectively. Through the expression of red fluorescent protein in the dual vector system, we can monitor the change of red fluorescent signal of the recombinant vector to test the performance of XOR gate.

Characterization

We used three different promoter combinations with XOR gate-mRFP to characterize the performance of the promoter and TP901-coupled logic gates, as well as the effect of the addition of lysis genes.

Characterization details can be found at：

pCadC-TP901: BBa_K4156099

pCadC-TP901-φ174E: BBa_K4156100

pLldR-TP901: BBa_K4156104

pLldR-TP901-φ174E: BBa_K4156105

pPepT-TP901: BBa_K4156108

pPepT-TP901-φ174E: BBa_K4156109


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 104
    Illegal AgeI site found at 1097
    Illegal AgeI site found at 1209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 124
    Illegal BsaI.rc site found at 351