Difference between revisions of "Part:BBa K4169029"

Revision as of 01:34, 12 October 2022

Mutated TMADH

After expressing, it'll produce trimethylamine dehydrogenase (TMADH (EC 1.5.99.7)). The enzyme TMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of trimethylamine (TMA) to dimethylamine and formaldehyde:
(CH₃)₃N + H₂O → (CH₃)₂NH + CH₂O +2H⁺ + 2e^-.

Metabolic Pathway

This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein. It couldn't work extracellular isolated.

Figure 1.Pathways for trimethylamine metabolism in bacteria.

Protein Molecular Structures

Trimethylamine dehydrogenase (TMADH) exist as dimers.

Figure 2.Protein molecular structures of trimethylamine dehydrogenase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 388
Illegal XhoI site found at 1717
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 388
Illegal PstI site found at 183
Illegal PstI site found at 1782
Illegal AgeI site found at 879
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4169029 short</partinfo>
+After expressing, it'll produce trimethylamine dehydrogenase (TMADH (EC 1.5.99.7)). The enzyme TMADH is an iron–sulfur flavoprotein which catalyses the oxidative demethylation of trimethylamine (TMA) to dimethylamine and formaldehyde: <br>(CH<sub>3</sub>)<sub>3</sub>N + H<sub>2</sub>O → (CH<sub>3</sub>)<sub>2</sub>NH + CH<sub>2</sub>O +2H<sup>+</sup> + 2e<sup>-</sup>.
-<!-- Add more about the biology of this part here
+==Metabolic Pathway==
-===Usage and Biology===
+<p>
+This enzyme is a complex iron-sulfur flavoprotein that transfers electrons to the soluble flavoprotein known as electron transferring flavoprotein. It couldn't work extracellular isolated.
+</p>
-<!-- -->
+<html>
+<div class = "center"><center><img src = "https://static.igem.wiki/teams/4169/wiki/backword/dma/trimethylamine-matabolism/tma-meta.png" style = "width:50%"></center><br></div>
+</html>
+<center><b>Figure 1.</b>Pathways for trimethylamine metabolism in bacteria. </center>
+<br>
+==Protein Molecular Structures==
+<p>
+Trimethylamine dehydrogenase (TMADH) exist as dimers.
+</p>
+<html>
+<div class = "center"><center><img src = "https://static.igem.wiki/teams/4169/wiki/backword/dma/tmd-dmd-structure/v344c-com.png" style = "width:75%"></center><br></div>
+</html>
+<center><b>Figure 2.</b>Protein molecular structures of trimethylamine dehydrogenase. </center>
+<br>
+===Sequence and Features===
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K4169029 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K4169015 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K4169029 parameters</partinfo>
 <!-- -->