Difference between revisions of "Part:BBa K4275002:Design"
| Line 18: | Line 18: | ||
<i>Neocallimastix patriciarum</i> W5 | <i>Neocallimastix patriciarum</i> W5 | ||
| + | <i>Clostridium thermocellum</i> (Dockerin-I domain) | ||
===References=== | ===References=== | ||
1.Chen, Hsin-Liang et al. "A Highly Efficient Β-Glucosidase From The Buffalo Rumen Fungus Neocallimastix Patriciarum W5". Biotechnology For Biofuels, vol 5, no. 1, 2012. Springer Science And Business Media LLC, https://doi.org/10.1186/1754-6834-5-24. | 1.Chen, Hsin-Liang et al. "A Highly Efficient Β-Glucosidase From The Buffalo Rumen Fungus Neocallimastix Patriciarum W5". Biotechnology For Biofuels, vol 5, no. 1, 2012. Springer Science And Business Media LLC, https://doi.org/10.1186/1754-6834-5-24. | ||
Revision as of 01:27, 12 October 2022
NpaBGS-t
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 502
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1577
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1074
Illegal AgeI site found at 88
Illegal AgeI site found at 106 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
1. The catalytic domain and the dockerin domain is interspaced with a glycine-rich linker.
2. The 79 amino acid long type-I dockerin domain is fused at the terminal of the GS linker, followed by a TEV site and a 8xhis affinity purification tag (HHHHHHHH).
3. DNA sequence is codon-optimized based on the codon-usage table of E.coli Strain K12.MG1655, but the protein was later expressed in K.marxianus due to the lack of post-translational modifications and the formation of inclusion bodies in E.Coli BL21(DE3).
Source
Neocallimastix patriciarum W5 Clostridium thermocellum (Dockerin-I domain)
References
1.Chen, Hsin-Liang et al. "A Highly Efficient Β-Glucosidase From The Buffalo Rumen Fungus Neocallimastix Patriciarum W5". Biotechnology For Biofuels, vol 5, no. 1, 2012. Springer Science And Business Media LLC, https://doi.org/10.1186/1754-6834-5-24.