Difference between revisions of "Part:BBa K4147012"

Latest revision as of 00:57, 12 October 2022

Up part of cassette for siRNA production of RXLR gene for P. capsici

Upper part of the cassette for siRNA expression. This part is part of the construct expressing the siRNA flanking the mRNA of the RXLR gene of P. capsici. It is cmpossed of a reverse T7 terminator with a RBS (BBa_K4147015) and a T7 promotor and RBS (BBa_K525998).

Usage and Biology

To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. For this method of construct assembly for dsRNA expression, we were inspired by the method used by the Ecuador 2021 team. When analyzing the possible options to produce siRNA we found that it was possible to use assembly methods to make constructs that encode in both directions.

The figure below shows the assembly strategy that is planned to be used.

Assembly strategy for siRNA production

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-To produce the double-stranded siRNAs, an expression system has been designed and must be assembled.
+To produce the double-stranded siRNAs, an expression system has been designed and must be assembled. For this method of construct assembly for dsRNA expression, we were inspired by the method used by the Ecuador 2021 team. When analyzing the possible options to produce siRNA we found that it was possible to use assembly methods to make constructs that encode in both directions.
-The assembly method is based on the one shown by the <b>iGEM Ecuador 2021 team</b>.
 The figure below shows the assembly strategy that is planned to be used.