Difference between revisions of "Part:BBa K4165230"

Latest revision as of 00:42, 12 October 2022

Clamp [15]

Clamp [15] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGGG (BBa_K4165019), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.

Usage and Biology

According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

3D Modeling and Ranking

TRRosetta models this composite part with a score of 5 out of 6 according to our quality assessment code (you can find the python script file on the programming club page with further explanation of how you can optimize it to your needs).

                              Figure 1. The 3D structure of Clamp 15 modeled by TRrosetta

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4165230 short</partinfo>
-Clamp [15] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGG (BBa_K4165018), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
+Clamp [15] composed of Tau Binding Peptide TD28rev (BBa_K4165006) , linker length of GGSGGGGG (BBa_K4165019), Tau Binding Peptide WWW (BBa_K4165007). Our Clamp design was based on our project aim for binding with Tau and amyloid beta for further degradation.
 ===Usage and Biology===
-According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of with various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).
+According to Previous Research results, Our binding peptides proved to have dual effect of binding to both Tau and amyloid beta . According to this , we designed our clamps consists of various linker lengths and peptides parts for further validation of this theory and analysis by wet-lab and dry lab results. According to our project Criteria , our clamps supposed to have binding affinity for tau and amyloid beta higher enough to make the inhibitor (BBa_K4165008) , (BBa_K4165010) unbind from catalytic domain of HTRA1 protease system (BBa_K4165004).
 <!-- -->