Difference between revisions of "Part:BBa K4377006:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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To verify the self-assembling ability of UBX protein, we created a composite part K4377006. (Figure 1. ) In order to purify our protein after expression, we added 10x His tag at the N terminal of Ubx protein. The reason we add 10x His tag at N terminal but not C terminal is to amplify the production of Ubx protein. To improve performance, we performed codon harmonization. The optimized sequence of codons will be more suitable for expression by our engineered ''E. coli''.
 
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====Cloning result====
 
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We successfully amplified His UBX plasmid in E.coli  Dh5α and transformed into E.coli BL21 for expression.
 
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====SDS-PAGE result====
 
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After expression, we conducted SDS PAGEs to verify whether the protein was produced.( result in Figure 3.)
 
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===Source===
 
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Drosophila melanogaster, sequence found through Uniprot https://www.uniprot.org/uniprotkb/P83949/entry
 
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===References===
 

Latest revision as of 00:03, 12 October 2022


Ultrabithorax (Ubx)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 208
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 421
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes