Difference between revisions of "Part:BBa K4160016"

 
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<partinfo>BBa_K4160016 short</partinfo>
 
<partinfo>BBa_K4160016 short</partinfo>
  
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This composite part combines BBa_K4160006 and BBa_K1470004.
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<p>This composite part encodes for STAT-induced SEAP and is composed of the basic parts STAT promotor (<a href="https://parts.igem.org/Part:BBa_K4160006">BBa_K4160006</a>) and SEAP (<a href="https://parts.igem.org/Part:BBa_K4160004">BBa_K1470004</a>).</p><br>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4160016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4160016 SequenceAndFeatures</partinfo>
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<h2>Usage & biology</h2>
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<a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a> used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.<sup>1</sup> Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of <a href="https://2022.igem.wiki/tu-eindhoven/">TU-Eindhoven 2022</a>, SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (<a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a>, <a href="https://parts.igem.org/Part:BBa_K4160009">BBa_K4160009</a>, <a href="https://parts.igem.org/Part:BBa_K4160010">BBa_K4160010</a>, <a href="https://parts.igem.org/Part:BBa_K4160012">BBa_K4160012</a>, and <a href="https://parts.igem.org/Part:BBa_K4160013">BBa_K4160013</a>). STAT-induced SEAP was expressed in a pLS13 plasmid.</p><br>
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<h2>Characterization </h2>
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<p>Characterization of STAT-induced SEAP expression by the GEMS system can be found on the <a href="https://parts.igem.org/Part:BBa_K4160008">BBa_K4160008</a> page.</p><br>
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<h2>References</h2>
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<p><small>
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<li>Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z</li>
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Revision as of 23:32, 11 October 2022


STAT-induced SEAP

This composite part encodes for STAT-induced SEAP and is composed of the basic parts STAT promotor (BBa_K4160006) and SEAP (BBa_K1470004).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 209
    Illegal PstI site found at 53
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 209
    Illegal BamHI site found at 227
    Illegal BamHI site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 209
    Illegal XbaI site found at 233
    Illegal PstI site found at 53
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1100
    Illegal BsaI.rc site found at 1589


Usage & biology

TU-Eindhoven 2022 used this composite part as an output of the Generalized Extracellular Molecule Sensor (GEMS) system that is developed by Scheller et al., 2018.1 Upon activation of the GEMS receptor, STAT-induced SEAP will be expressed and secreted via the JAK/STAT signaling pathway. In the project of TU-Eindhoven 2022, SEAP was used as a genetic reporter protein to investigate the activation of the GEMS receptor (BBa_K4160008, BBa_K4160009, BBa_K4160010, BBa_K4160012, and BBa_K4160013). STAT-induced SEAP was expressed in a pLS13 plasmid.


Characterization

Characterization of STAT-induced SEAP expression by the GEMS system can be found on the BBa_K4160008 page.


References

  1. Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M. Generalized extracellular molecule sensor platform for programming cellular behavior. Nat Chem Biol. Published online 2018. doi:10.1038/s41589-018-0046-z