Difference between revisions of "Part:BBa K4201006"

 
 
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<partinfo>BBa_K4201006 short</partinfo>
 
<partinfo>BBa_K4201006 short</partinfo>
  
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Description
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Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the yew tree (genus <i>Taxus</i>). We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast<sup>1</sup>, which would require us to add export tags and make our engineering goal more difficult to reach.  Taxadiene Synthase II, a variation of taxadiene synthase from the Chinese yew has been found to have the highest output of paclitaxel out of any plant mode, and is more effective in paclitaxel biosynthesis then the more well-known, Pacific Yew-derived taxadiene synthase<sup>2</sup>.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:20, 11 October 2022


cytoTDS2-MBP

Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the yew tree (genus Taxus). We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast1, which would require us to add export tags and make our engineering goal more difficult to reach. Taxadiene Synthase II, a variation of taxadiene synthase from the Chinese yew has been found to have the highest output of paclitaxel out of any plant mode, and is more effective in paclitaxel biosynthesis then the more well-known, Pacific Yew-derived taxadiene synthase2.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 974
    Illegal PstI site found at 661
    Illegal PstI site found at 2222
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 661
    Illegal PstI site found at 2222
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 974
    Illegal PstI site found at 661
    Illegal PstI site found at 2222
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 974
    Illegal PstI site found at 661
    Illegal PstI site found at 2222
    Illegal AgeI site found at 810
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 3566